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I am analyzing a data set of frogs photographed at night with flash. Using the 3% and 19% gray standards from at DKC-Pro White Balance & Color Calibration Chart that was photographed after each picture in the same location to make mspec files. My problem is this: No matter whether the ROI is a green frog, a brown frog, bright green duckweed, dark green algae, brown wet log or gray dry tree bark – the R values are always greater than the G values than the B values (R>G>B). I feel something has to be wrong here, but I can’t figure out what it is. Any suggestions?

R>G>B in all ROI measures
Cedric van den Berg Changed status to publish November 6, 2025