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Hello! I am interested in determining how post-transplant seedling color affects survival. I just need a way to quantify how “red” vs “brown” vs “green” a seedling is relative to the others. This program seems to be perfect in that it can standardize the images based on a grayscale and give me an RGB of the leaves I select, but I am confused about what to do with the output. The RGB values I am getting are between zero and maybe 25, which all seem far too dark for the conventional 0-255 RGB scale. Squaring the values seems to help, but it goes beyond 255 if the value is above like 15. May I please have some guidance on how to interpret the RGB outputs it gives me? Thank you for your help on what might be an obvious question!

How to interpret RGB values for Mspec image?
Cedric van den Berg Changed status to publish August 5, 2025