I’m using ImageJ to quantify reflectance of melanin coloration and extent of melanin coloration (using basic thresholding). I’m new to this plug in and multispectral images. Can I do simple tresholding with multispectral images? If so, how?
But yes, you can simply create a linear normalised, or cone-catch image and then use ImageJ’s comprehensive thresholding tool. There’s also an adaptive thresholding tool in the toolbox that might be useful if there are low-spatial frequency elements to ignore (e.g. shadows).