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I am trying to calculate the uv reflectance of an area of an insect in a black and white photo. There are a 98% and a 4% standard in the photo. As far as I’m aware I have to create a mspec photo to find the UV reflectance value. When I do this I use a ‘UV’ camera setting that I made so only red and blue channels are shown. So when I measure UV reflectance I get different mean uv reflectance values for the two channels, whereas I want one uv value from the image. How do I do this ?

Calculating uv reflectance of a black and white image
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