Hi all – We are trying to produce color maps of lizards using the “RNL Colour Maps” –> “1 – Create RNL Colour Maps from ROIs” function. We have done this before (in 2020), using the same images, and it worked perfectly. We haven’t changed any of our settings (to my memory). Now, it’s producing color maps that are white shapes on black backgrounds. I can supply an example of an image if you like. Any suggestions? Thanks!
Without knowing more: The problem might be related to the .mspec images having been created under mica 2.1 but using mica 2.2 on them? Specifically, it could be due to the change in how the intensity of the channels is coded (bits in 2.1 vs. % in 2.2). If so, recreating the .mspec files in 2.2. might fix the issue?