I would like to measure the amount of black spotting on white eggshells. I know how to create an mspec image with an roi on the egg but it is still unclear to me how to best measure the black spotting. I watched the videos on youtube and the Naive Bayes looks promising but I am unsure if that is the best method for what I am trying to do.
More specific direction would be extremely helpful. I know how to do particle analysis by just using ImageJ but I would like to be able to use Mica.
Yes, you could use either clustering method and then look at the transition matrix (where the proportion of synonymous transitions approximate the % of each cluster) or the other outputs such as the exactly said particle analysis which will give you a precise pixel and %area value.